![]() ![]() As an aggressive tumor type of ovarian cancer, HGSOC had a higher relapse rate (~25%) and poorer overall 5-year survival rate (31%). Serous ovarian cancer is a gynecological tumor that is more common in women, and high-grade serous ovarian cancer (HGSOC) accounts for roughly 70% of ovarian cancer deaths. It is possible to generate novel potential targeted therapy strategy and translational application for serous ovarian carcinoma patients with a predictable better clinical outcome. Together, these results revealed that CtBP1/2 play a different regulatory role in genomic stability and DSBs repair pathway bias in serous ovarian cancer cells. Interesting, blast through TCGA tumor cases, patients with CtBP2 genetic alternation had a significantly longer overall survival time than unaltered patients. Furthermore, CtBP1/2 knockdown shifted the DSBs repair pathway from homologous recombination (HR) to non-homologous end joining (NHEJ) and activated DNA-PK in SKOV3 cells. CtBP1 protects the integrity of stalled forks under metabolic stress condition during prolonged periods of replication, whereas CtBP2 acts a dominant role in stability of DNA replication recovery. The results of DNA fiber assay revealed that CtBP1/2 contribute differentially to the integrity of DNA replication track and stability of DNA replication recovery. CtBP1/2 knockdown induced cancer cell apoptosis, increased genetic instability, and enhanced the sensitivity to DNA damage agents, such as γ-irradiation and chemotherapy drug (Carboplatin and etoposide). Several key functional pathways were significantly enriched through whole transcription profile analysis of CtBP1/2 knockdown SKOV3 cells, including DNA damage repair, apoptosis, and cell cycle. In this study, we combined whole-transcriptome analysis with multiple research methods to investigate the role of CtBP1/2 in genomic stability. However, little is known about CtBP proteins’ regulatory roles in genomic stability and DNA repair in HGSOC. CtBP proteins were identified with overexpression in the high-grade serous ovarian carcinoma (HGSOC). Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.The C-terminal binding proteins (CtBPs), CtBP1 and CtBP2, are transcriptional co-repressor that interacts with multiple transcriptional factors to modulate the stability of chromatin. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. *These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.  Lane 1: Prime-Step⢠Prestained Broad Range Protein Ladder Lane 2: Competitor 1 protein ladder Lane 3: Competitor 2 protein ladder.įor research use only. Proteins were either stained with Coomassie blue, or transferred to nitrocellulose membrane followed by Ponceau red staining. Supplied in the sample loading buffer and is ready to useģµl Prime-Step⢠Prestained Broad Range Protein Ladder was resolved by electrophoresis in different gel running systems: 4-20% Tris-Glycine gel, 4-12% Bis-Tris gel (MES running buffer).Better performance for high molecular weight proteins.Comparable MW detection with competitorâs protein ladders.Suitable for size detection during protein purification. ![]() Excellent transfer efficiency on PVDF and Nitrocellulose membrane for WB.Clear separation and sharp bands in different SDS-PAGE gel and buffer systems.The Prime-Step⢠Prestained Broad Range Protein Ladder is designed for monitoring protein separation during SDS-PAGE electrophoresis, confirming Western blot transfer quality on membranes (PVDF or nitrocellulose membrane) and for approximating the size of target proteins on the gel or blot. The Prime-Step⢠Prestained Broad Range Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 6.5 to 270 kDa. It has 7 blue-stained bands and 3 reference bands (2 orange bands at 30 kDa and 270 kDa and 1 green band at 52 kDa) when separated on Tris-glycine gel. Protein ladder, protein marker, pre-stained protein ladder, pre-stained protein marker, protein molecular weight marker ![]()
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